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IFG-1 reconstitution

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Mr_Gargan
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2008/05/20 17:41:44 (permalink)
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IFG-1 reconstitution

Hello everyone, sorry if this question is already answered elsewhere but I've been searching around for ages now.

I've just bought some IGF-1 and its come in single vials, 1000mcg. I've read that I need to dilute the IGF with Benzyl Alcohol then draw out the amount to be used and further dilute with bacteriostatic water or sterile water. Does this sound right? If not what should I do? I've heard about freezing pins?

If Benzyl Alcohol is the way forward can anyone point me in the direction of a supplier, because like I said, i've been searching these boards endlessly.

Thanks everyone
#1

5 Replies Related Threads

    thecodehaseyes
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    RE: IFG-1 reconstitution 2008/05/20 18:49:12 (permalink)
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    Welcome mate.

    Im not going to go into too much detail, but basically:

    Reconstiution of IGF-1 in 1000mcg vials are primarily done with Acetic Acid 100mM. This is because it appears the only solution that keeps the IGF stable long enough. Once reconstituted you can then dilute loaded syringe with Bac water to reduce the sting from the AA.

    Note: You could use BA, but ive not seen a study on the stability duration using it solo.

    Reconstituting IGF - Using Distilled White Vinegar for Acetic Acid

    I began using this method many months ago ... mainly out of impatience. I was out of Benzyl Alcohol, and while I had access to HCl, it wasn't accessible when I wanted to begin my Long R3 IGF-1 cycle. I decided to use plain ol' grocery store Distilled White Vinegar. I used the following, which has worked out really well for me....and the feedback I have had from others that I have shared it with has been positive so far. So here it is for anyone that is interested:

    The calculation:
    Distilled white vinegar is supposed to be standardized to ~5% acetic acid, which would make it 850mM. To get it to the recommended 100mM, you'd want 11.76% white vinegar (100mM/850mM = 11.76%). Since it would be almost impossible to draw out 11.76IU's, I round this to 12, which is certainly going to be close to our desired 100mM.

    The filtering process:
    I use off the shelf grocery store distilled white vinegar. In order to ensure safety, I filter it using .20u whatman filters. Here is the step by step for those that may not be familiar with filtering using whatmans. What you will want to have on hand before starting out is some sterile vials, some .20u whatman filters, some syringes and needles (I use a 10cc syringe, and .23 gauge 1" needles), and some alcohol swabs.

    (1) First draw up about 10cc of the distilled white vinegar
    (2) screw on the .20u whatman to the 10cc syringe (or whatever size you use)
    (3) screw on a .23 gauge needle (or whatever size you decide to use)
    (4) take your sterile vial, swab the top with alcohol, insert a needle for venting.
    (5) Insert your syringe/whatman/needle apparatus and slowly push the 10cc's into the sterile vial.

    Now you have safe vinegar to use for your reconstituting.

    ALTERNATE METHOD - Alternately, you could simply mix your water and distilled white vinegar before filtering using about 7.5 parts of water per 1 part of distilled white vinegar. After mixing these together in this ratio, run the mixture through your .20u whatman as above. You will end up with a vial of dilutent this way that has the proper PH for use with your IGF-1.

    Reconstituting:

    How much water/vinegar you reconstitute with is going to somewhat depend on which LR3 IGF-1 you are using. Igtropin is shipped in 100mcg vials, which I usually reconstitute at 1ml(cc) per 100mcg vial (which will make the 10 mark on your insulin syringe = 10 mcgs). The gropep based IGF-1's are primarily shipped in 1mg vials, and I usually use 5ml for these (which will make the 10 mark on your insulin syringe = 20mcgs).
    At any rate, what I do is:

    (1) take an alcohol swab and swab the tops of my water, vinegar solution, and IGF-1 vials
    (2) take a syringe with a 23 gauge, 1" needle and draw out .12 cc's of vinegar for the 100mcg vials or .60 cc's for the 1mg vials.
    (3) next I take this syringe and draw out the water - .88cc's for 100mcg, 4.4cc's for the 1mg.

    FOR ALTERNATE METHOD in lieu of steps (2) and (3) - Just draw out the desired amount of dilutent from your pre-mixed vial of
    vinegar / water.

    (4) next i poke the needle into the LR3 IGF-1 vial and dribble this solution down the side of the vial, avoid any direct spray on the lyophilized powder until all of the dilutent is in the vial
    (5) using a gentle swirling motion, I reconstitute the powder.
    (6) I stick the vial in the fridge and it is now ready for use.

    Well, I think that about sums it up. Hope this helps anyone who may have been wondering about using vinegar to reconstitute. I would advise that if you end up using Igtropin, you seriously consider using this vinegar method. Igtropin and other dilutents such as BA do not get along well together at all.

    RedBaron @ IFL

    Bench Press -200kg for 1
    Dumbell Seated Press -2 x 60kg for 6
    #2
    Mr_Gargan
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    RE: IFG-1 reconstitution 2008/05/21 00:16:00 (permalink)
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    Thanks pal, that's very informative. Can you point me in the direction of a supplier of these .20u whatman filters?

    Much appreciated
    post edited by Mr_Gargan - 2008/05/21 00:25:24
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    Mr_Gargan
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    RE: IFG-1 reconstitution 2008/05/21 00:33:51 (permalink)
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    OK, I just did a few searches and they cost a fortune and come in boxes of 100 from what I gather. Is there no way I could get hold of a couple cheaply? Someone must have a few laying around...

    Am I even allowed to ask on here?
    #4
    redneil75
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    RE: IFG-1 reconstitution 2008/05/21 08:44:00 (permalink)
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    dont need them. Reconstitute with the acetic acid, then keep in fridge, or if not possible wrap in tin foil and keep in a thermos flask as i do. Draw the solution 1st then draw enough bac water to fill syringe, and bobs yer art garfunkle!
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    Mr_Gargan
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    RE: IFG-1 reconstitution 2008/05/23 20:32:01 (permalink)
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    ALTERNATE METHOD - Alternately, you could simply mix your water and distilled white vinegar before filtering using about 7.5 parts of water per 1 part of distilled white vinegar. After mixing these together in this ratio, run the mixture through your .20u whatman as above. You will end up with a vial of dilutent this way that has the proper PH for use with your IGF-1.


    The water used, is that sterile water? Bact-water? tap water? I've got Bact-water - that suitable?

    Cheers
    #6
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