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IGF-1 Info

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2005/07/25 10:07:16 (permalink)

IGF-1 Info

Does anyone have any good websites with info on IGF-1 and bodybuilding? I'm having trouble getting info on it...
post edited by PartyBoy - 2005/07/25 10:20:30

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    RE: IGF info 2005/07/25 10:14:25 (permalink)
    Courtesy of Lawnsaver at Article is a couple of years old so some info may be outdated as more is becoming known:

    R3(long) IGF-1

    1: Type- IGF-1 Long R3 (Anything else is not as effective, and if the person providing it for you doesn't know anything about it, you are asking for trouble.)
    2. Storage- the most popular (and most effective) way to store, transport, preserve IGF is by suspending it in sterile BA in a sterile vial.
    This will keep your IGF 99% potent for many months at a time in just about ANY indoor storage, I.E.-closet, drawer, etc. (Take it from me, I stored mine because I wasn't ready to use it for about 6 months in my closet... I had fears about its potency, then I started my first week, and BAM I practically cleaned out the fridge.
    3. Use- Usage should not exceed 4-5 weeks, and an OFF period should be about the same. Daily dosages work best (split up into 2 seems to make little difference in the Long R3 version) Most people see results at about 40mcg/day, some use as low as 30mcg/day, and some folks even use 80-100mcg. I SUGGEST to ALL first time users no matter what level, to start at about 40-50mcg/day.
    4. Administration- I believe in IM injections over sub q, but either seems to be effective. I like IM better because IM using a slin pin is probably the least painful thing one could imagine, even at two times per day. Also, sub Q shots that contain BA, even diluted BA, can leave little nodules that you may not want to feel on your stomach.
    5. Mixing- Most IGF comes suspended in BA. Hopefully it is @ 500mcg/ml or even 333mcg/ml (that would be at 2ml/mg and 3ml/mg respectively) Draw out your desired amount and back load a slin pin. Add enough bacteriostatic Water to fill the U100 syringe completely.
    Some inject immediately before training, while others choose to do 2 shots spread throughout the day... THEY BOTH WORK WELL. Try both; see which method makes your muscles pop out of your skin.
    6. Add plenty of protein, and don’t shy away from carbs immediately after training. I used up to 100g of carbs after training, and my body fat went down, all without cardio.

    I hope that helps a little, and I’m glad to be lurking around this board again.
    More to come.


    The most effective form of IGF is Long R3 IGF-1, it has been chemically altered and has had amino acid changes, which cause it to avoid binding to proteins in the human body and allow it to have a much longer half-life, around 20-30 hours. "Long R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substation of an Arg(R) for the Glu (E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. This analog of IGF-1 has been produced with the purpose of increasing the biological activity of the IGF peptide."

    "Long R3 IGF-1 is significantly more potent than IGF-1. The enhanced potency is due to the decreased binding of Long R3 IGF-1 to all known IGF binding proteins. These binding proteins normally inhibit the biological actions of IGF's."

    It is also not as expensive since a media grade version is available which is sufficient for bodybuilding use. There is also a receptor grade available but it is VERY expensive and the only noticeable difference between the two would only be able to be noticed in a laboratory setting. The price on the black market for Long R3 IGF-1 can be seen anywhere from $300-$500 per milligram depending on the source, be wary of black market dealers of any IGF since it is a VERY difficult item to obtain. As mentioned IGF is a research product and is only available from a few laboratories in the world and is only available to research companies and biotechnology institutions. For the rest of this article when I say IGF I am now referring to Long R3 IGF-1 for simplicity sake.

    Any form of IGF is ONLY supplied in a lyophilized form, which means a dry powder state. NEVER PUCHASE PRE-DILUTED LIQUID IGF!!!! There is no such product made anywhere in the world and even if there were real IGF ever present in the vial it would all be dead by the time you receive it. IGF is a very delicate peptide and must be diluted by yourself, where you have access to a refrigerator and freezer. There has also been a lot of talk by certain sources claiming to have IGF made by the Eli Lilly company, to clear things up Lilly is a pharmaceutical company and as stated IGF is a research drug and has not yet been approved, Lilly does not and never has manufactured research drugs for retail sale.

    The diluents you will need for the IGF are a weak concentration of hydrochloric acid and a sterile buffer (sterile water or bacteriostatic water) the procedure for diluting the IGF is not very difficult, the diluents can be obtained from most local chemical suppliers and a good source of IGF would also be able to supply the necessary diluents.

    The most effective length for a cycle of IGF is 50 days on and 20-40 days off. The most controversy surrounding Long R3 IGF-1 is the effective dosage. The most used dosages range between 20mcg/day to 120+mcg/day. IGF is only available by the milligram, one mg will give you a 50 day cycle at 20mcg/day, 2mg will give you a 50 day cycle at 40mcg/day, 3mg will give you a 50 day cycle at 60mcg/day, 4mg will give you a 50 day cycle at 80mcg/day and so on. The dosage issue mainly revolves around how much money you have to spend, plenty of people use the minimum dosage of 20mcg/day and are happy with the results, and in fact several top bodybuilders use the 20mcg/day dosage and are pleased with the results. IGF is most effective when administered subcutaneous and injected once or twice daily at your current dosage. The best time for injections is either in the morning and/or immediately after weight training.

    Another frequently asked question of IGF refers to the real world results; in terms of pure weight gain don't expect to gain 5 lbs. a week like you may off of anadrol or a similar steroid. The only weight you will gain from IGF use is pure lean muscle tissue, with steroids most of the weight gained is water weight. With an effective dosage you can expect to gain 1-2 lbs of new lean muscle tissue every 2-3 weeks and these effects can be increased with the use of testosterone, anabolic steroids, and insulin use. Increased vascularity is also very common, people report seeing veins appear where they never have before. And yet another effect reported is the ability to stay lean while bulking with heavy dosages of steroids and TONS of food while on an IGF cycle, this is perhaps the most pleasing effect. Increased pumps are also noticeable almost immediately, the pumps can almost become painful, and pumps are even noticeable when doing cardio.

    Overall, IGF is a very exciting drug due to its ability to alter ones genetic capabilities. If you can find a trustworthy source and you use it correctly it can be a VERY useful tool in your bodybuilding drug arsenal.

    Insulin-Like Growth Factor Recombinant 3
    IGF-1 R3
    IGF1 stands for insulin like growth factor. It mimics insulin in the human body and also at the same time makes the muscles more sensitive to insulin’s effects. It is a growth factor and is the most potent one in the human body at that. IGF causes muscle cell hyperplasia, which is an actual splitting and forming of new muscle cells. This was thought to only be possible during puberty. IGF is much more potent at this effect than growth hormone is, in fact almost all of the effects you see from growth hormone come from the increased amount of IGF that your liver produces when the GH is destroyed. So it would be very easy to say that IGF is a much more potent and cheaper alternative to GH use, although GH is more effective for fat loss than IGF due to some other effects that it causes such as metabolism increase and the ability to effectively use more insulin, t3, and anabolic steroids.
    Another advantage that IGF has over GH is that it has much more of an affinity to attach to muscle cells instead of bone and organ cells. Growth hormone has been know to cause a lot of organ enlargement and bone elongation since it attaches to all types of receptor cells. IGF is much more likely to go where we want it, our muscle cells. IGF-1 attaches to myogenic stem cells, which are only located in muscle and connective tissues. These myogenic stem cells are responsible for the production of myoblast cells, which in turn are responsible for the buildup and repair of connective tissues (ligaments, tendons, cartilage, and joints to a certain extent).
    So from this you can see that IGF-1 is great for increasing the strength of tendons and also for helping to heal existing injuries while at the same time helping to prevent them. IGF-1 is also responsible for increased protein synthesis and amino acid synthesis.
    IGF does not have to be used along with anabolic steroids, GH, insulin, or thyroid hormones to be effective. It causes muscle growth on its own. In fact some people prefer to use it during their breaks from steroid cycles since IGF has no effect on natural test production. It could effectively be used along with HCG, Clomid, and PGF2a for a hell of an off cycle stack which would allow your body to return to normal and still allow you to grow!! On its own IGF will give an increase of around 2 lbs. of new solid lean muscle tissue every two weeks, and is also is know for its ability to strip off body fat and GREATLY increase vascularity, body fat decreases of 5-8% over a 50 day cycle are not uncommon. But, of course you will be much happier with the results if you use the IGF along with anabolic steroids, testosterone, and insulin.
    The use of steroids along with the IGF allow you to quickly mature and strengthen the new muscle tissue that the IGF has formed, and may also speed the process of hyperplasia. If you need any help setting up a great stack to
    use along with the IGF just let me know and I can help you out. I speak with lots of top bodybuilders and guru’s so I am very knowledgeable.
    The dosage issue for IGF is where the most controversy lies. Dosages used by competitive athletes most commonly range anywhere between 60mcg/day to 100+mcg/day. The trick is finding the dosage that works best for YOU. For most the best results appear when you reach a dosage of 80mcg/day, while some do
    receive good results from only 40mcg/day. I personally feel the best results begin to be noticed at a dosage of 100mcg/day. I personally am using 150mcg/day during my current cycle.
    Also I should let you know that the form of IGF is the Long R3 analog. It has been chemically altered and has a longer half-life than regular IGF, which only lasts about 10 minutes in the human body once injected. The Long R3 IGF-1 has a half-life of 6-10 hours, so you will only need to inject once or twice per day. The best time to inject is after lifting and in the morning, so it would be best to use half the dosage in the morning and the other half after lifting. This will take maximal advantage of IGF’s insulin
    mimicking effects.
    post edited by PartyBoy - 2005/07/25 10:20:03

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    RE: IGF info 2005/07/25 10:18:26 (permalink)
    Another profile courtesy of JohnnyB:

    LongR3 IGF-I

    The efficient and effective manufacture of recombinant proteins, antibodies, vaccines and viral products in animal cells requires a source of growth factors or some means of growth factor signaling to cells. The traditional method is to provide an external source of growth factors, primarily through the addition of fetal bovine serum to culture media. However, many sectors of the biotechnology
    and pharmaceutical industries are now demanding pure recombinant growth factors, made under the highest quality standards, or inclusion in serum-free media formulations.

    The insulin-like growth factors (IGFs) were originally discovered and purified from serum. They are considered to be important growth factors for industrial cell culture because:

    • They are present in all animal and human sera at concentrations of 100 - 500 µg/L.

    • The removal of IGFs from serum can abolish up to 90% of the cell growth-promoting activity.

    • They stimulate nutrient uptake, cell growth, protein synthesis and inhibit apoptosis or programmed cell death in a wide range
    of cell types.

    • Almost all cells have type I IGF receptors, which mediate the biological action of IGFs.

    Many industrially important cell types can be cultured in serum-free media that contain high concentrations of insulin (5 - 10 mg/L). This is about 1000-fold higher than the normal physiological concentration of insulin. Insulin only works in cell culture because it acts as a weak substitute for IGFs. Much lower levels of IGFs can replace insulin.

    The insulin-like growth factors are structurally related to insulin. There are two forms, IGF-I and IGF-II, which are similar and have closely related actions on cell growth via the same receptor. IGF-I is considered to be the main post-natal growth-promoting factor and IGF-II has major effects during fetal development. IGF-I is a non-glycosylated, single chain polypeptide 70 amino acids in length.

    Structure of IGF-I and Insulin
    Insulin-like growth Factor-I Insulin

    IGF-I is similar in structure to pro-insulin, the precursor of insulin. Pro-insulin is a single chain polypeptide, which is cleaved to remove the connecting C peptide, to form insulin. Insulin has two chains (A and B chain) connected by two disulphide bonds.

    The receptors for IGF-I and insulin are also structurally related and both ligands interact with each other’s receptors with very low affinity. In cell culture, the potency of IGF-I is higher than insulin because the cellular responses required for biopharmaceutical production in animal cells are mediated via the type I IGF receptor, not the insulin receptor.

    Another important feature is that a family of six IGF binding proteins regulates the biology of IGF peptides. These proteins are found in serum and are also produced by cells in culture. IGF binding proteins bind IGFs with high affinity and generally inhibit the actions of IGFs on cells. This has been exploited by making analogs of IGF peptides that do not bind to IGF binding proteins and are therefore superior to both IGF-I and insulin in cell culture. The most potent of these analogs for commercial cell culture purposes is LongR3 IGF-I.

    LongR3 IGF-I
    LongR3I GF-I has been specifically engineered and manufactured by GroPep Limited for use in serum-free cell culture media. Structurally it has two significant modifications — first, one amino acid in the IGF-I structure, the glutamic acid (E) at position 3 has been replaced with an arginine (R), which accounts for the R3 in the name; and second, because the molecule is made as a fusion protein, it has an N-terminal fusion partner which is 13 amino acids long. Thus the “LongTM” in the name.

    Structure of LongR3 IGF-I

    Replacing the glutamic acid (E) with arginine (R) at position 3 is important because this modification significantly reduces the binding of the growth factor to the IGF-I binding proteins, enabling LongR3 IGF-I to be so potent. The addition of the fusion partner also enhances refolding and facilitates high-yield production. The end result is a growth factor 10-fold more potent in cultured cells compared to native IGF-I and 200- to 1000-fold more potent than insulin.

    A general comparison of properties related to potency in cell culture is provided in the following table.

    LongR3I GF-I is manufactured in genetically engineered E. coli. The manufacturing process uses no animal sourced material, making it regulatory friendly for commercial biopharmaceutical production. The system of production is briefly outlined below:

    1. Fermentation. E. coli containing the gene for LongR3 IGF-I are grown in a fermenter. GroPep
    Limited’s patented expression system uses inclusion body technology.

    2. Homogenization. Bacteria are lysed to release inclusion bodies that are harvested by differential

    3. Dissolution. The recombinant fusion protein is released into solution. It is not correctly folded
    into its tertiary protein structure at this point.

    4. Refolding. The LongR3 IGF-I protein is incubated under controlled conditions so that the
    disulphide bonds can correctly form to allow the correct protein structure. The protein would
    be biologically inactive or less active in an incorrectly folded form.

    5. Purification. A four-step system is used to purify LongR3 IGF-I. This series of steps also incorporates accepted protocols for the removal of bacterial endotoxin.

    6. Supply. The product is subjected to quality control assays and is available as a lyophilized
    powder or can be manufactured as a liquid for delivery to customers.

    It is important to be aware that insulin is acting in cell culture systems as a weak analog of IGF-I.
    LongR3I GF-I will therefore work in any serum-free medium or cell culture system in which insulin is used. The potency compared to insulin is best illustrated by the data published by Morris, et al, 20001. They found that LongR3 IGF-I was superior to insulin in terms of recombinant protein production, primarily by increasing the number of viable Chinese Hamster Ovary (CHO) cells in a small production system. LongR3 IGF-I was used in the µg/L range compared to insulin in the mg/L concentration range.

    Advantages of LongR3 IGF-I

    There are several advantages to using LongR3 IGF-I in cell culture rather than insulin.
    1. LongR3 IGF-I is Better Cell Science. Because LongR3 IGF-I acts directly on the type I IGF
    receptor it is the right tool for the job. And since far less LongR3 IGF-I is required in media than
    insulin it can make downstream processing easier and more efficient as well.

    2. LongR3 IGF-I Outperforms Insulin. Published research has shown that LongR3 IGF-I leads to overall greater productivity by increasing cell viability and delaying programmed cell death.

    3. LongR3 IGF-I is Readily Available and Regulatory Friendly. Since LongR3 IGF-I is a recombinant protein manufactured in a process without any animal-derived components it eliminates regulatory concerns. It is a proven cell culture product currently employed in the manufacturing process
    of a number of FDA-approved biopharmaceuticals. A secure and ample manufacturing capacity
    ensures a continual, ready supply for commercial production needs.

    4. LongR3 IGF-I is Less Expensive than Insulin. Depending on the amount of LongTMR3IGF-I used to achieve cell growth, and the productivity enhancements one achieves, LongR3 IGF-I can be
    significantly less expensive than insulin on a dollar/liter basis as shown on the chart below. Also,
    over time, as LongR3 IGF-I usage increases, the cost of production will decrease – making it even
    less expensive, while insulin costs have been steadily increasing.

    Preparation and use of LongR3 IGF-I

    LongR3 IGF-I is supplied as a freeze-dried formulation or as a liquid.

    The freeze-dried formulation is packed in an atmosphere of nitrogen at a slight vacuum. To prepare a solution for cell culture, introduce an air filled syringe through the septum to equalize the pressure. Next, add sufficient 10 mM HCl or 100 mM acetic acid to the vial to achieve a peptide concentration of at least 0.1 mg/mL. Concentrations of 1 mg/mL or more are recommended. Mix the solution thoroughly to ensure the peptide is completely dissolved. The solution can then be filtered through a low-protein binding membrane before addition to cell culture medium or it can be added directly to the medium, which can subsequently be filtered.

    The liquid product is formulated in acetic acid (100 mM) at a concentration of 5 - 7 g/L and is ready to dilute straight into cell culture medium to achieve a biologically active concentration of about 50 µg/L. The final dilution of 100,000-fold, means that there is no effect on pH or osmolality of the cell culture medium.

    A titration for LongR3 IGF-I should be performed for each different application as the optimum concentration may vary slightly depending upon the cell type used and other components present in the medium. The recommended final concentration range of LongR3 IGF-I is 10 to 50 µg/L.

    Because LongR3 IGF-I and insulin act through the same cell receptor, the effectiveness of LongR3 IGF-I will be masked if it is added in conjunction with commonly employed concentrations of insulin (~10 mg/L). However, inclusion of physiological levels of insulin (~5 µg/L) in cell culture medium containing the recommended levels of LongR3 IGF-I can result in beneficial synergistic effects in certain applications.

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    RE: IGF info 2005/07/25 10:19:39 (permalink)
    PLEASE NOTE: I have not had time to research IGF-1 R3 at this time so cannot offer much in the way of corrobaration at this juncture.

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    RE: IGF info 2005/07/25 22:26:57 (permalink)
    Thanks for the articles... u got anything on the side effects??
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